El pescado en la comedia griega

Presentado en el Congreso Internacional "Sociedad, política y literatura. Comedia griega antigua", organizado por la Universidad de Salamanca y celebrado en Salamanca los días 26 a 30 de noviembre de 1996.

Relación entre el índice de consumo de pescado y carne y la adecuación y calidad de la dieta en mujeres jóvenes universitarias

9 p.

Parodia épica y gastronomía: el Ἀττικὸν Δεῖπνον de Matrón de Pítane

[ES] En la antigua Grecia existió una floreciente literatura de tema gastronómico, poco conocida porque sólo se ha conservado una pequeña parte de ella. Uno de los escasos ejemplos que conservamos es el Ἀττικὸν Δεῖπνον de Matrón de Pítane, que, por medio de la parodia de los poemas homéricos, describe un pantagruélico banquete en el que predominan los platos de pescado. El anfitrión, los comensales, incluso los alimentos servidos adoptan las maneras de los héroes y los dioses, creándose un profundo contraste entre la forma seria y el contenido ligero, con el único propósito de divertir.

Resveratrol prevents inflammation-dependent hepatic melanoma metastasis by inhibiting the secretion and effects of interleukin-18

Background: Implantation and growth of metastatic cancer cells at distant organs is promoted by inflammation-dependent mechanisms. A hepatic melanoma metastasis model where a majority of metastases are generated via interleukin-18-dependent mechanisms was used to test whether anti-inflammatory properties of resveratrol can interfere with mechanisms of metastasis. Methods: Two experimental treatment schedules were used: 1) Mice received one daily oral dose of 1 mg/kg resveratrol after cancer cell injection and the metastasis number and volume were determined on day 12. 2) Mice received one daily oral dose of 1 mg/kg resveratrol along the 5 days prior to the injection of cancer cells and both interleukin-18 (IL-18) concentration in the hepatic blood and microvascular retention of luciferase-transfected B16M cells were determined on the 18(th) hour. In vitro, primary cultured hepatic sinusoidal endothelial cells were treated with B16M-conditioned medium to mimic their in vivo activation by tumor-derived factors and the effect of resveratrol on IL-18 secretion, on vascular cell adhesion molecule-1 (VCAM-1) expression and on tumor cell adhesion were studied. The effect of resveratrol on melanoma cell activation by IL-18 was also studied. Results: Resveratrol remarkably inhibited hepatic retention and metastatic growth of melanoma cells by 50% and 75%, respectively. The mechanism involved IL-18 blockade at three levels: First, resveratrol prevented IL-18 augmentation in the blood of melanoma cell-infiltrated livers. Second, resveratrol inhibited IL-18-dependent expression of VCAM-1 by tumor-activated hepatic sinusoidal endothelium, preventing melanoma cell adhesion to the microvasculature. Third, resveratrol inhibited adhesion-and proliferation-stimulating effects of IL-18 on metastatic melanoma cells through hydrogen peroxide-dependent nuclear factor-kappaB translocation blockade on these cells. Conclusions: These results demonstrate multiple sites for therapeutic intervention using resveratrol within the prometastatic microenvironment generated by tumor-induced hepatic IL-18, and suggest a remarkable effect of resveratrol in the prevention of inflammation-dependent melanoma metastasis in the liver.

Vascular endothelial growth factor regulates melanoma cell adhesion and growth in the bone marrow microenvironment via tumor cyclooxygenase-2

Background: Human melanoma frequently colonizes bone marrow (BM) since its earliest stage of systemic dissemination, prior to clinical metastasis occurrence. However, how melanoma cell adhesion and proliferation mechanisms are regulated within bone marrow stromal cell (BMSC) microenvironment remain unclear. Consistent with the prometastatic role of inflammatory and angiogenic factors, several studies have reported elevated levels of cyclooxygenase-2 (COX-2) in melanoma although its pathogenic role in bone marrow melanoma metastasis is unknown. Methods: Herein we analyzed the effect of cyclooxygenase-2 (COX-2) inhibitor celecoxib in a model of generalized BM dissemination of left cardiac ventricle-injected B16 melanoma (B16M) cells into healthy and bacterial endotoxin lipopolysaccharide (LPS)-pretreated mice to induce inflammation. In addition, B16M and human A375 melanoma (A375M) cells were exposed to conditioned media from basal and LPS-treated primary cultured murine and human BMSCs, and the contribution of COX-2 to the adhesion and proliferation of melanoma cells was also studied. Results: Mice given one single intravenous injection of LPS 6 hour prior to cancer cells significantly increased B16M metastasis in BM compared to untreated mice; however, administration of oral celecoxib reduced BM metastasis incidence and volume in healthy mice, and almost completely abrogated LPS-dependent melanoma metastases. In vitro, untreated and LPS-treated murine and human BMSC-conditioned medium (CM) increased VCAM-1-dependent BMSC adherence and proliferation of B16M and A375M cells, respectively, as compared to basal medium-treated melanoma cells. Addition of celecoxib to both B16M and A375M cells abolished adhesion and proliferation increments induced by BMSC-CM. TNF alpha and VEGF secretion increased in the supernatant of LPS-treated BMSCs; however, anti-VEGF neutralizing antibodies added to B16M and A375M cells prior to LPS-treated BMSC-CM resulted in a complete abrogation of both adhesion-and proliferation-stimulating effect of BMSC on melanoma cells. Conversely, recombinant VEGF increased adherence to BMSC and proliferation of both B16M and A375M cells, compared to basal medium-treated cells, while addition of celecoxib neutralized VEGF effects on melanoma. Recombinant TNFa induced B16M production of VEGF via COX-2-dependent mechanism. Moreover, exogenous PGE2 also increased B16M cell adhesion to immobilized recombinant VCAM-1. Conclusions: We demonstrate the contribution of VEGF-induced tumor COX-2 to the regulation of adhesion-and proliferation-stimulating effects of TNFa, from endotoxin-activated bone marrow stromal cells, on VLA-4-expressing

Three-dimensional growth as multicellular spheroid activates the proangiogenic phenotype of colorectal carcinoma cells via LFA-1-dependent VEGF: implications on hepatic micrometastasis

Background: The recruitment of vascular stromal and endothelial cells is an early event occurring during cancer cell growth at premetastatic niches, but how the microenvironment created by the initial three-dimensional (3D) growth of cancer cells affects their angiogenesis-stimulating potential is unclear. Methods: The proangiogenic profile of CT26 murine colorectal carcinoma cells was studied in seven-day cultured 3D-spheroids of <300 mu m in diameter, produced by the hanging-drop method to mimic the microenvironment of avascular micrometastases prior to hypoxia occurrence. Results: Spheroid-derived CT26 cells increased vascular endothelial growth factor (VEGF) secretion by 70%, which in turn increased the in vitro migration of primary cultured hepatic sinusoidal endothelium (HSE) cells by 2-fold. More importantly, spheroid-derived CT26 cells increased lymphocyte function associated antigen (LFA)-1-expressing cell fraction by 3-fold; and soluble intercellular adhesion molecule (ICAM)-1, given to spheroid-cultured CT26 cells, further increased VEGF secretion by 90%, via cyclooxygenase (COX)-2-dependent mechanism. Consistent with these findings, CT26 cancer cells significantly increased LFA-1 expression in non-hypoxic avascular micrometastases at their earliest inception within hepatic lobules in vivo; and angiogenesis also markedly increased in both subcutaneous tumors and hepatic metastases produced by spheroid-derived CT26 cells. Conclusion: 3D-growth per se enriched the proangiogenic phenotype of cancer cells growing as multicellular spheroids or as subclinical hepatic micrometastases. The contribution of integrin LFA-1 to VEGF secretion via COX-2 was a micro environmental-related mechanism leading to the pro-angiogenic activation of soluble ICAM-1-activated colorectal carcinoma cells. This mechanism may represent a new target for specific therapeutic strategies designed to block colorectal cancer cell growth at a subclinical micrometastatic stage within the liver.

Desarrollo de métodos de detección de salmonella basados en la reacción en cadena de la polimerasa y su validación en muestras alimentarias

224 p.

La cerámica de la Segunda Edad del Hierro en el País Vasco: Estudio tecnológico, funcional y social

670 p. Capítulos de introducción, metodología, discusión y conclusiones en castellano e inglés.

Control de vuelo de un cuadricóptero AR-Drone

[ES]En este TFG se obtendrá un algoritmo de generación de trayectorias para el cuadricóptero AR-Drone capaz de conseguir trazados lo más rápidos posibles teniendo en cuenta la dinámica del modelo y las limitaciones del entorno. Así mismo se propondrá un control primitivo para la detección de obstáculos. Mediante las comunicaciones con el cuadricóptero y el control de los parámetros característicos del medio, se establecerá usando el lenguaje C# los bucles de programación necesarios para discriminar todas las trayectorias desfavorables y obtener una que se adecue a nuestros criterios.

Asesinos seriales: etiología de sus crímenes y perfilación

[ES]El objetivo del presente trabajo es aproximarse a la realidad del fenómeno del asesinato serial desde las diferentes perspectivas, teorías y autores. Para ello, en un principio, se hace alusión a los debates existentes sobre la definición del mismo y las tipologías de asesinos seriales. Después de esto, se pretenden estudiar las controversias surgidas sobre la etiología de sus crímenes y los factores que afectan a la comisión de estos. Además, en base a la tipología de este tipo de asesinos expuesta por parte del FBI, se hará referencia a su psicopatología y a su perfil criminal.

Planta de cogeneración de 5 MWe con MACI para una fábrica de conservas

Estudio sobre la implantación de un sistema de cogeneración en una fábrica de conservas de pescado, que utilizaba una caldera convencional a gas natural y desea aprovechar al máximo la energía de la que dispone

Proyecto Ondarroa S.A-ren biziraupenerako estrategiak

Euskera: Gradu Amaierako Lan honetan Proyecto Ondarroa enpresaren kasu azterketa azaltzen da. Proyecto Ondarroa 2008an Ondarroako portuko hainbat armadorek sortu zuten arrain banaketan aritzen den banaketa enpresa da, lehen salmentan erosle, zuzenean itsasontzietatik erosten ditu izakinak gero banakuntzan aritzeko balioa erantsi ondoren. Analisi estrategikoa egin da, gero enpresaren epe luzeko biziraupenaren estrategiak garatzeko. Horrela, ikusiko dugu 2008an arrantza krisi eta ekonomia krisiari Ondarroako portuko hainbat enpresariek erantzun zioten. Ordutik hona portuko egoera eta arrantzaleen egoera aldatu egin da, zirkunstantzia hauetan bizirautea geroz eta zailagoa bihurtuz.